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KMID : 1094720110160050992
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Biotechnology and Bioprocess Engineering
2011 Volume.16 No. 5 p.992 ~ p.992
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Antioxidant activity of the halophyte Limonium tetragonum and its major active components
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Lee Jung-Im
Kong Chang-Suk
Jung Myoung-Eun
Hong Joo-Wan
Lim Sun-Young
Seo Young-Wan
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Abstract
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In this study, the antioxidant potentials of crude extracts and solvent-partitioned fractions of Limonium tetragonum were assessed by measuring their ability to scavenge intracellular reactive oxygen species (ROS) generated in HT-1080 cells. Following activity-oriented separation, four flavonol glycosides were isolated as active principles and their chemical structures were determined by 2 D NMR and by comparison with reported spectral data. The isolated compounds (1?4) were evaluated for their antioxidant capacity using three different activity tests; degree of occurrence of intracellular ROS, lipid peroxidation in HT-1080 cells and the extent of oxidative damage of genomic DNA purified from HT-1080 cells. All compounds exhibited significantly inhibited the generation of intracellular ROS and lipid peroxidation in HT-1080 cells, and significantly inhibited DNA oxidation. In addition, direct free radical scavenging effects of these compounds were investigated using the electron spin resonance (ESR) spin-trap technique.
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KEYWORD
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Limonium tetragonum, antioxidant activity, reactive oxygen species, ESR
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